earle’s balanced salt solution (ebss (Thermo Fisher)
Structured Review

Earle’s Balanced Salt Solution (Ebss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/earle+balanced+salt+solution+ebss/pmc11801289-59-6-11
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer’s disease in vitro and in vivo"
Article Title: Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer’s disease in vitro and in vivo
Journal: Neural Regeneration Research
doi: 10.4103/NRR.NRR-D-23-01633
Figure Legend Snippet: VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: Earle’s Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
Techniques Used: In Vitro, Confocal Microscopy, Fluorescence
Related Articles
other:Article Title: Biallelic SQSTM1 mutations in early-onset, variably progressive neurodegeneration Article Snippet: In all experiments evaluating autophagy, we starved cells in Incubation:Article Title: Targeting the Dependence on PIK3C3-mTORC1 Signaling in Dormancy-Prone Breast Cancer Cells Blunts Metastasis Initiation Article Snippet: .. For amino acid starvation, cells were first serum-starved overnight, then rinsed with PBS, and incubated in |