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earle’s balanced salt solution (ebss  (Thermo Fisher)


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    Thermo Fisher earle’s balanced salt solution (ebss
    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: <t>Earle’s</t> Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
    Earle’s Balanced Salt Solution (Ebss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/earle+balanced+salt+solution+ebss/pmc11801289-59-6-11
    Average 90 stars, based on 1 article reviews
    earle’s balanced salt solution (ebss - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer’s disease in vitro and in vivo"

    Article Title: Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer’s disease in vitro and in vivo

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-23-01633

    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: Earle’s Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
    Figure Legend Snippet: VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: Earle’s Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.

    Techniques Used: In Vitro, Confocal Microscopy, Fluorescence

    Related Articles

    other:

    Article Title: Biallelic SQSTM1 mutations in early-onset, variably progressive neurodegeneration
    Article Snippet: In all experiments evaluating autophagy, we starved cells in Earle balanced salt solution (EBSS; Gibco, Thermo Fisher Scientific, Waltham, MA), as specified.

    Incubation:

    Article Title: Targeting the Dependence on PIK3C3-mTORC1 Signaling in Dormancy-Prone Breast Cancer Cells Blunts Metastasis Initiation
    Article Snippet: .. For amino acid starvation, cells were first serum-starved overnight, then rinsed with PBS, and incubated in Earle balanced salt solution (EBSS; Thermo Fisher Scientific, #24010043) for 2 hours at 37°C. ..



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    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: <t>Earle’s</t> Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
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    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: <t>Earle’s</t> Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
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    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: <t>Earle’s</t> Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
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    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: <t>Earle’s</t> Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
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    Thermo Fisher earle’s balanced salt solution ebss #24010043
    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: <t>Earle’s</t> Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
    Earle’s Balanced Salt Solution Ebss #24010043, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher earle’s balanced salt solution (ebss)
    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: <t>Earle’s</t> Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
    Earle’s Balanced Salt Solution (Ebss), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: Earle’s Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.

    Journal: Neural Regeneration Research

    Article Title: Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer’s disease in vitro and in vivo

    doi: 10.4103/NRR.NRR-D-23-01633

    Figure Lengend Snippet: VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: Earle’s Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.

    Article Snippet: Then, the cells were treated with Earle’s Balanced Salt Solution (EBSS) (Thermo Fisher Scientific) for 4 hours to induce autophagy or Bafilomycin A1 (Baf A1, MedChem Express, Monmouth Junction, NJ, USA) for 4 hours to inhibit autophagy.

    Techniques: In Vitro, Confocal Microscopy, Fluorescence